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Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media
Used for capture and intermediate purification requiring low to medium hydrophobicity
Supplier: Cytiva 17096510
Description
- Base matrix derivatized via uncharged, chemically-stable ether linkages
- Two levels of substitution help find optimal selectivity and binding capacity for given application
- Hydrophilic nature of the base matrix ensures low levels of non-specific binding
- Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
- Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
- Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
- Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel
Specifications
25 mL | |
Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea | |
Phenyl Sepharose 6 Fast Flow Media (low sub) | |
45 to 165 μm | |
3 to 13 | |
Phenyl |
For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity | |
4°C to 30°C | |
250 to 400 cm/Hoμr | |
6% Cross-Linked Agarose | |
20% Ethanol |
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