GE Healthcare PreScission Protease
Use for site-specific separation of the GST tag from proteins expressed using pGEX-6P vectors. GE Healthcare Life Sciences PreScission Protease G allows simultaneous protease immobilization and cleavage of GST fusion proteins.
Manufacturer: GE Healthcare 27084301
- Description: A genetically engineered fusion protein consisting of human rhinovirus 3C protease and GST; Specifically cleaves between the Gln and Gly residues of the recognition sequence of LeuGluValLeuPheGln/GlyPro.
- Application: Facilitates removal of the protease by allowing simultaneous protease immobilization and cleavage of GST fusion proteins produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3
- Storage Buffer Components: 50mM Tris-HCl (pH 8.0), 150mM NaCl, 10mM EDTA, 1mM DTT, and 20% glycerol.
- Unit Definition: One unit will cleave >90% of 100µg of a test GST-fusion protein in 50mM Tris-HCl, 150mM NaCl, 1mM EDTA, 1mM DTT, pH 7.0 at 5°C for 16 hrs.
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|Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8; Cleavage Buffer: 50mM Tris HCl pH 8, 150mM NaCl, 10mM EDTA, 1mM DTT and 20% Glycerol|
|Fusion Protein Protease|
|Facilitates removal of the protease by allowing simultaneous protease immobilization and cleavage of >90% of 100μg of a test GST-fusion protein produced from the pGEX-6P vectors pGEX-6P-1, pGEX-6P-2 and pGEX-6P-3|
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