Cytiva Protein A and Protein G Mag Sepharose™

Simplify enrichment of target proteins by immunoprecipitation or pull-down applications

Catalog Number	Mfr. No.	Bead Type	Binding Capacity	Ligand	Quantity
45-002-161	Cytiva 28944006	Protein A Mag Sepharose	8 to 17mg Human IgG/mL	Native Protein A	500 μL
45-002-160	Cytiva 28944008	Protein G Mag Sepharose	13 to 22mg Human IgG/mL	Protein G	500 μL
45-002-173	Cytiva 28951379	Protein G Mag Sepharose	13 to 22mg Human IgG/mL	Protein G	4 x 500 μL
45-002-174	Cytiva 28951378	Protein A Mag Sepharose	8 to 17mg Human IgG/mL	Native Protein A	4 x 500 μL

Description

Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

• Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
• Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
• Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
• Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C

Specifications

• Matrix: Paramagnetic spherical, highly cross linked agarose particles
• Particle Size: 37 to 100 μm
• Content And Storage: 4°C to 8°C