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GE Healthcare Protein G HP MultiTrap™ Plates

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates

Manufacturer:  GE Healthcare 28903135

Catalog No. 45-001-486


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Includes: Four prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

Description

Description

  • Flexible format, with protocols for both classic elution and cross-link methods
  • Formatted for elution for both electrophoresis and LC-MS analysis workflows
  • Fast-binding kinetics and high capacity provide high yield
  • Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
  • Can be used with centrifugation or vacuum, manually or in automated robotic systems
  • Easy scale-up with HiTrap™ Protein G HP prepacked columns

  • Matrix: Highly cross-linked agarose, 6%
  • Binding capacity: approx. 25mg human IgG/mL medium (0.5 to 1mg IgG/well)
  • Average particle size: 34µm
  • pH stability: 3 to 9 (long term); 2 to 9 (short term)
  • Well volume: 800µL
  • Centrifugation force: <700 x G
  • Vacuum pressure: -0.5 bar (max.)

Specifications

Specifications

Protein G Sepharose HP MultiTrap plate
Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked 96-well filter plates that are ready to use, electrophoresis or LC/MS
Polypropylene, Polyethylene
-0.5 bar
34μm
2° to 8°C
4 Pack
4 Prepacked Protein G HP MultiTrap 96-well filter plates with instructions, plus a proto col
Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
3 to 9 (long term), 2 to 9 (short term)
6% Highly Cross-linked Agarose
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