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Cytiva Protein G HP SpinTrap™ Columns Available on GSA/VA Contract for Federal Government customers only.

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

Manufacturer:  Cytiva 28903134

Catalog No. 45-001-485

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Includes: 16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.



  • Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
  • Formatted for elution for both electrophoresis and LC-MS analysis workflows
  • Fast-binding kinetics and high capacity provide high yield
  • Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
  • Easy scale-up with HiTrap™ Protein G HP prepacked columns

  • Matrix: Highly cross-linked agarose, 6%
  • Binding capacity: >1mg human IgG/column
  • Average particle size: 34µm
  • pH stability: 3 to 9 (long term); 2 to 9 (short term)
  • Column volume: 800µL
  • Max. sample loading volume: 600µL

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Protein G Sepharose HP SpinTrap spin column
Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS
Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
4° to 8°C
16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col
3 to 9 (long term), 2 to 9 (short term)
6% Highly Cross-linked Agarose


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