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GE Healthcare Ready-to-Go™ You-Prime First-Strand Beads

Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. GE Healthcare Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.

Manufacturer:  GE Healthcare 27926401

Catalog No. 45-001-416


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Includes: Fifty preformulated, single-dose first-strand reaction mix beads prepackaged in white, thin-walled 0.5mL tubes compatible with most thermal cyclers. Each tube contains two beads containing buffer, dATP, dCTP, dGTP, dTTP, murine reverse transcriptase, RNase inhibitor, and RNase/DNase-free BSA. Also includes five control mix beads packaged in red tubes containing globin mRNA, buffer, and two globin-specific PCR primers for evaluating the performance of the Reaction Mix Beads and the PCR amplification.

Description

Description

  • Predispensed, ambient-temperature-stable, single-dose reaction beads minimize pipetting errors, avoid cross-contamination, and ensure optimal performance
  • Each first-strand reaction requires only the addition of RNA in DEPC-treated water and a user-selected first-strand primer
  • First-strand reaction may be primed with oligo(dT) primers, random primers such as pd(N)6, or custom primers complementary to a specific mRNA sequence
  • Conditions of this reaction have been optimized to permit full-length transcription of RNAs 7kb or more in length
  • Final reaction volume is 33µL once reconstituted
  • Ideal for research applications that use PCR to detect and quantify eukaryotic RNA from a variety of samples

Specifications

Specifications

The kit provides 50 preformulated, single-dose reaction beads prepackaged in thin-walled 0.5 mL tubes compatible with most thermal cyclers. For synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice.
Fifty preformulated, single-dose first-strand reaction mix beads prepackaged in white, thin-walled 0.5mL tubes compatible with most thermal cyclers, each tube contains two beads containing Buffer, dATP, dCTP, dGTP, dTTP, murine reverse transcriptase, RNase inhibitor and RNase/DNase-free BSA
50 reactions
First-Strand
Ready-To-Go You-Prime First-Strand Beads
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