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GE Healthcare rmp Protein A Sepharose™ Fast Flow Affinity Media

Designed for high-purity separation of monoclonal and polyclonal antibodies

Manufacturer:  GE Healthcare 17513801

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Catalog No. 45-002-974


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Description

Description

  • Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
  • Coupling chemistry that minimizes leakage of protein A ligand
  • Multi-point attachment of rProtein A by chemically-stable amide bonds
  • High capacity and good pressure/flow characteristics provide for high throughput

  • Matrix: 4% cross-linked agarose
  • Average particle size: 90µm (d50V)
  • Ligand: recombinant protein A (E. coli)
  • pH Stability working range: 3 to 10
  • pH Stability cleaning-in-place: 2 to 11
  • Binding capacity/mL chromatography medium: >35mg human IgG/mL
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column

For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.

Specifications

Specifications

>Min. 22 mg Human IgG/mL
For High-Purity Separation of Monoclonal and Polyclonal Antibodies
25 cm
4% Cross-Linked Agarose
5 mL
20% Ethanol
60 - 165 µm
3 to 10
150 to 250 cm/Hoµr
9.84 in.
Recombinant Protein A (E. coli)
100 kPa
Stable in aqueous Buffers commonly used in Protein A Chromatography
2°C to 8°C
rmp Protein A Sepharose fast flow media
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