GE Healthcare rmp Protein A Sepharose™ Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Designed for high-purity separation of monoclonal and polyclonal antibodies

Manufacturer: GE Healthcare 17513801

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Catalog No. 45-002-974

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    Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
    Quantity 5mL
    Storage Requirements 2 to 8°C
    Binding Capacity >Min. 22mg Human IgG/mL
    Product Type rmp Protein A Sepharose fast flow media
    For Use With (Application) For high-purity separation of monoclonal and polyclonal antibodies
    Flow Rate 150 to 250cm/Hour
    Particle Size 60 to 165μm
    Height (English) Bed 9.84 in.
    Height (Metric) Bed 25cm
    Max. Pressure 100kPa
    Matrix 4% Cross-Linked Agarose
    pH Range 3 to 10
    Storage Buffer 20% Ethanol
    Ligand Type Recombinant Protein A (E. coli)

    • Non-mammalian-based media composed of recombinant Protein A coupled with Sepharose 4 Fast Flow base matrix by reductive amination
    • Coupling chemistry that minimizes leakage of protein A ligand
    • Multi-point attachment of rProtein A by chemically-stable amide bonds
    • High capacity and good pressure/flow characteristics provide for high throughput

    • Matrix: 4% cross-linked agarose
    • Average particle size: 90µm (d50V)
    • Ligand: recombinant protein A (E. coli)
    • pH Stability working range: 3 to 10
    • pH Stability cleaning-in-place: 2 to 11
    • Binding capacity/mL chromatography medium: >35mg human IgG/mL
    • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column

    For therapeutic applications requiring extremely pure eluate fractions of antibodies with non-exposure of antibodies to human or animal derivatives during purification process.

  • Description & Specifications

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