GE Healthcare rProtein A Sepharose™ Fast Flow Affinity Media Available on GSA/VA Contract for Federal Government customers only.

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Manufacturer: GE Healthcare 17127901

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Catalog No. 45-002-971

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    Stability Stable in aqueous Buffers commonly used in Protein A Chromatography
    Quantity 5mL
    Storage Requirements 2 to 8°C
    Binding Capacity 50mg Human lgG/mL medium2
    Product Type rProtein A Sepharose fast flow media
    For Use With (Application) For purifiCatIon of monoclonal and polyclonal antibodies at labs and process scales
    Flow Rate 150 to 250cm/Hour
    Particle Size 60 to 165μm
    Height (English) Bed 9.84 in.
    Height (Metric) Bed 25cm
    Max. Pressure 100kPa
    Matrix 4% Cross-Linked Agarose
    pH Range 2 to 9
    Storage Buffer 20% Ethanol
    Ligand Type Recombinant Protein A (E. coli)

    • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
    • High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
    • No mammalian components involved during manufacturing process
    • Matrix: 4% cross-linked agarose
    • Average particle size: 90µm (d50V)
    • Ligand: 6mg rProtein A/mL medium (E. coli)
    • Binding capacity: ∽50mg human IgG/mL media
    • pH stability working range: 3 to 10
    • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed

  • Description & Specifications

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