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GE Healthcare Sephacryl™ S-500 High Resolution Media Available on GSA/VA Contract for Federal Government customers only.

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Manufacturer:  GE Healthcare 17061310

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Catalog No. 45-002-053


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Description

Description

Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

  • For separating polysaccharides, macromolecules with extended structures, and small plasmids
  • Excellent purification over a wide molecular weight range
  • High reproducibility due to long-term chemical and physical stability
  • High flow rates and recoveries
  • Well-suited to industrial-scale use
  • Storage conditions: 20% ethanol; 4° to 30°C
  • Available in a variety of selectivities and volumes

Chemical Stability (stable in all common buffers):

  • 1M acetic acid, 8M urea
  • 6M guanidine-HCl, 1% SDS
  • 2M NaCl, 24% ethanol
  • 30% propanol, 30% acetonitrile
  • 0.5M NaOH (for cleaning)
pH Stability
  • 3 to 11 (long-term and working)
  • 2 to 13 (short-term)
Physical Stability:
  • Negligible volume variation due to changes in pH or ionic strength
  • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
  • Antimicrobial agent: 20% ethanol

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Specifications

Specifications

150mL
>125cm/hr.
Sephacryl S-500 HR media
40 to 140cm
5.9 in.
100kPa
3 to 11
Polysaccharides, macromolecules and small plasmids
4° to 30°C
19 to 230L
Solid Spherical Bead
Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
15cm
25 to 75μm
Allyl Dextran and N, N'-methylenebisacrylamide
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