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Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Manufacturer:  Cytiva 17009010

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Catalog No. 45-002-042

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  • Prepared for use in polar organic solvents and aqueous solvent mixtures
  • Made from hydroxypropylated dextran beads that have been crosslinked to yield a polysaccharide network
  • Hydrophilic and lipophilic dual nature of the matrix enables powerful chromatographic selectivity
  • Batch-to-batch reproducibility
  • Partical size dry: 18μ-111μm
  • Fractionation range globular proteins: 4x103 to 5 x 103

  • Stable in most aqueous and organic eluent systems
  • Shelf life: 5years
  • Autoclavable: 20 min at 121°C
  • Flow Velocity <∽12cm/min
  • pH stability working range: 2-13

Analytical and industrial scales for the preparation of closely related molecular species



Solid Spherical Bead
For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
18 to 111μm in dry form
Hydroxypropylated, cross-linked Dextran
4 to 30°C
Sephadex LH-20
11.81 in.
25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
2 to 13
Steroid, Terpenoid, Lipid, low molecular weight Peptide
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