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Cytiva (Formerly GE Healthcare Life Sciences) StrepTactin™ Sepharose™ High Performance Medium

Mild purification conditions preserve antigenicity and function. Cytiva StrepTactin Sepharose High Performance media is used for robust, high resolution purification of Strep II-tagged proteins.

Manufacturer:  Cytiva (Formerly GE Healthcare Life Sciences) 28935599

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Catalog No. 45-001-585

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  • Medium with high binding capacity yields high resolution, sharp concentrated peaks, and reproducible purifications
  • Physiological conditions and mild elution preserves the activity of the target protein
  • Compatible with a wide range of reducing agents, detergents, denaturants, and other additives
  • Easily scaled up, and easily regenerated with 0.5M NaOH
  • Convenient use with a peristaltic pump or chromatography system

  • Matrix: rigid, highly cross-linked 6% agarose
  • Ave. particle size: 34µm
  • Dynamic binding capacity (protein dependent): approx. 6mg Strep(II)-tagged protein/mL medium
  • Compatibility: stable in all commonly used buffers, 0.5M NaOH (regeneration and cleaning), reducing agents, denaturants, and detergents
  • pH stability: >7



StrepTactin Sepharose HP media
Purification of Strep (II) -tagged recombinant Proteins to high purities in concentrated forms and small volumes by using with perstaltic pump or chromato graphy system
StrepTactin (∼5mg StrepTactin/mL medium)
2° to 8°C
<300 cm/hr.
6% Highly Cross-linked Agarose
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