GE Healthcare Thrombin Protease
For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors
Manufacturer: GE Healthcare 27084601
- Supplied as lyophilized powder
- Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
- Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
- Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow
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|Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3|
|Thrombin Protease Fusion Protein|
|Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours|
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