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Cytiva (Formerly GE Healthcare Life Sciences) Thrombin Protease Available on GSA/VA Contract for Federal Government customers only.

For site-specific separation of the GST tag from proteins expressed using pGEX-T vectors

Manufacturer:  Cytiva (Formerly GE Healthcare Life Sciences) 27084601

Catalog No. 45-001-320

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  • Supplied as lyophilized powder
  • Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
  • Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
  • Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow

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Elution Buffer: 50mM Tris HCl, 10mM reduced Glutathione pH 8, Cleavage Buffer: PBS: 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4 pH 7.3
Thrombin Protease Fusion Protein
Site-specific separation of the GST tag from proteins expressed using pGEX-T vectors, one unit of enzyme cleaves 90% of 100μg of a test GST fusion protein when incubated in 1X PBS at 22°C for 16 hours


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