Thermo Scientific™ GelCode Blue Safe Protein Stain is a fast and sensitive coomassie-dye reagent that provides one-step ultrasensitive detection of proteins in polyacrylamide gels and is formulated to avoid hazardous shipping costs.GelCode Blue Safe Protein Stain is a Coomassie G-250 dye-based reagent for staining protein on polyacrylamide gels and membranes. GelCode Blue Safe Stain provides considerable sensitivity, allowing for the detection down to 9ng of protein. This protein-specific stain allows bands to be viewed directly on a gel or membrane during staining and is compatible with mass spectrometry applications. The staining process is simple and flexible, usually requiring a 15-minute incubation of the gel or sample with the GelCode Blue Safe Protein Stain, followed by a simple water wash to yield blue protein bands and a clear background. Because the supplied Activator Crystals are packaged and shipped dry in a separate bottle, GelCode Blue Safe Protein Stain is classified as nonhazardous under the U.S. Department of Transportation (DOT) shipping requirements.
Sensitive and fast – detect down to 9ng of protein per band in 15 minutes
Safe – noncorrosive to skin, nonflammable, and safe to ship and store until activated
Convenient – water washes only; no acid-fixative or methanol destain solutions required
Versatile – suitable for staining 1D and 2D polyacrylamide gels, nitrocellulose, and PVDF membranes (multiple protocols provided in the instructions)
Compatible – useful for qualitative visualization, quantitative densitometry, and downstream excision and analysis by mass spectrometry
Easy to use – add and dissolve activator crystals before first use, then simply add reagent to stain gels
Stable – store non-activated or activated stain at room temperature for up to one year
Safety and Handling
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