Gemin 2 Monoclonal Antibody (2E17), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA15777

Catalog No. 01-674-891

Description

MA1-5777 detects Gemin2 in human and xenopus samples. MA1-5777 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5777 detects a band of approximately 34 kDa. The MA1-5777 immunogen is His-Gemin2 (starting at 2nd methionine). Store at 4oC for one month, or in small aliquots at -20oC for longer periods.

SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

Specifications

• Antigen: Gemin 2
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: O14893, Q9CQQ4
• Gene Symbols: GEMIN2, gemin2.S
• Immunogen: His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, X. laevis
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: 2E17
• Conjugate: Unconjugated
• Gene: GEMIN2
• Gene Alias: 1700012N19Rik; Component of gems 2; gem (nuclear organelle) associated protein 2; gem nuclear organelle associated protein 2; gem nuclear organelle associated protein 2 S homeolog; gem-associated protein 2; GEMIN2; Gemin-2; gemin2.S; SIP 1; SIP1; SIP-1; SIP1 delta; sip1-delta; SMN interacting protein 1-delta; SMN interacting protein-1; SMN-interacting protein 1; survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1; survivor of motor neuron protein interacting protein 1; XELAEV_18042638mg
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 494588, 66603, 8487
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

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