Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Supplier:  Thermo Scientific™ K0731

Catalog No. FERK0731

Description

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
• Efficient—high yields of total RNA.
• Fast—15 minutes after lysis step
• Pure—an A260/280 ratio > 1.9
• Convenient—spin columns are capped and assembled with collection tubes

Applications
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
• RT-PCR
• RT-qPCR
• Northern blotting
• Nuclease protection assay

Includes
Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

Specifications

• Content And Storage: • 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
  • 40 mL Lysis Buffer; room temperature
  • 40 mL Wash Buffer 1 (concentrated); room temperature
  • 23 mL Wash Buffer 2 (concentrated); room temperature
  • 30 mL Nuclease-free Water; room temperature
  • 50 GeneJET RNA Spin Columns with Collection Tubes; room temperature
  • 50 Collection Tubes (2 mL); room temperature
  • 50 Collection Tubes (1.5 mL); room temperature
• Purification Time: 15 min. (After sample homogenization)
• Sample Type: Bacteria, Blood, Cells, Tissue, Yeast, Insects, Enzymatic Reactions
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Quantity: 50 Preps
• Shipping Condition: Room Temperature
• Yield: Up to 160 μg

• Isolation Technology: Silica Spin Column
• Elution Volume: 50–100 μL
• Final Product Type: Total RNA
• For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
• Scale: Mini
• Starting Material Amount: Bacteria: ≤10⁹ cells
  Blood: 0.5 mL
  Cells: ≤10⁷
  Tissue and Insects: 30 mg
  Yeast: ≤4 x 10⁸ cells
  Enzymatic reactions: ≤100 μL

For Research Use Only. Not for use in diagnostic procedures.