GFAP Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA110019

Catalog No. 01-670-276

Description

By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50 kDa corresponding to rodent GFAP and a lower band at ∽45 kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

Specifications

• Antigen: GFAP
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GFAP
• Gene Alias: 6330404F12Rik; 65 kDa glutamic acid decarboxylase; AI836096; ALXDRD; Astrocyte or Intermediate Filament Protein; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; GAD(65); Gad2; Gad-2; GAD65; GAD-65; GFAP; GFAP epsilon; gfap protein; gfapl; glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; glutamic acid decarboxylase 2; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Equine, Human, Mouse, Pig, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum with 5mM sodium azide
• Gene Accession No.: P03995, P14136, P47819, Q28115
• Gene Symbols: GFAP
• Immunogen: Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
• Regulatory Status: RUO
• Gene ID (Entrez): 100033970, 14580, 24387, 2670, 281189, 396562
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov