GFAP Rat anti-Bovine, Human, Mouse, Rat, Alexa Fluor® 647, Clone: 2.2B10, eBioscience
Rat Monoclonal Antibody
Manufacturer: eBioscience 51979282
Description: This 2.2B10 monoclonal antibody recognizes human, mouse, rat, and bovine glial fibrillary acid protein (GFAP). The immunogen used to generate this antibody was enriched bovine glial filaments. Applications Reported: This 2.2B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 2.2B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product No. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red LaserGFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavi ly and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|P03995, P14136, P47819, Q28115|
|Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein|
|4° C, store in dark, DO NOT FREEZE!|
|14580, 24387, 2670, 281189|
|Alexa Fluor™ 647|
|Enriched bovine glial filaments|
|Bovine, Human, Mouse, Rat|
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