Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Gibco™ Advanced DMEM/F-12 Non-distribution product as customer accommodation.

Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ Advanced DMEMF/12 is used in cell culture processing applications.

Manufacturer:  Gibco™ 12634028

 View more versions of this product

Catalog No. 12-634-028


  Check Availability
Add to cart

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.


Requires : Supplementation

Specifications

Description

  • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
  • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
  • Contains no proteins, lipids, or growth factors
  • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
  • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
  • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
Description & Specifications

Specifications

Mammalian cell culture
AlbuMAX II, Ammonium Metavanadate, Ascorbic Acid phosphate, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ethanolamine, Ferric Nitrate (Fe(NO3)3·9H2O), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glutathione monosodium, Glycine, Human Transferrin (Holo), Hypoxanthine Na, Insulin Recombinant Full Chain, L-Alanine, L-Arginine hydrochloride, L-Asparagine-H2O, L-Aspartic acid, L-Cysteine hydrochloride-H2O, L-Cystine 2HCl, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (anhydrous), Magnesium Sulfate (MgSO4) (anhyd.), Manganous Chloride, Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Reducing Agents, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Sodium Selenite, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
DMEM/F-12 media
Storage: 2-8° C. Protect from light
L-glutamine, HEPES
Liquid
Sustainable packaging
10 x 500mL
Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red

Safety and Handling

ShelfLife : 12 months from date of manufacture

Promotions

Promotions

  • Invitrogen , Gibco
    Buy up to $3,000 of Thermo Fisher Scientific™ Life Sciences products and get the same amount to redeem for more Life Sciences products. This is an NLSU exclusive offer. You must apply and qualify for the program to be eligible.

    06-30-2018

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit