Gibco™ DMEM, High Glucose, GlutaMAX™ Supplement, HEPES Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Dulbecco's Modified Eagle Medium is widely used basal medium for supporting growth of many different mammalian cells

Manufacturer: Gibco™ 10564029

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Catalog No. 10-564-029

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When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File. For human ex vivotissue and cell culture processing applications

Required Accessories : Supplementation


    For Use With (Application) Mammalian cell culture
    Format Liquid
    Formulation Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Ferric Nitrate (Fe(NO3)3·9H2O), Folic Acid, Glycine, L-Alanyl-Glutamine, L-Arginine hydrochloride, L-Cystine, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4) (anhyd.), Niacinamide, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Sodium Pyruvate, Thiamine hydrochloride, i-Inositol
    Packaging Sustainable packaging
    Product Type DMEM media
    Quantity 10 x 500mL
    Shelf Life 12 months from date of manufacture
    Storage Requirements Storage: 2-8° C. Protect from light
    With Additives High Glucose, HEPES, GlutaMAX™, Phenol Red
    Without Additives Sodium Pyruvate

    • Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
    • Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
    • Contains no proteins, lipids, or growth factors
    • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
    • Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
    • Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12

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    Shipping Condition: Room Temperature

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