Freestyle™ CHO Expression Medium Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Animal origin-free, chemically defined, protein-free medium

Manufacturer: Gibco 12651014

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Catalog No. 12-651-014

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    Specifications

    Cell Line CHO, FreeStyle™ CHO-S Cells
    Additive No glutamine, no phenol red
    Format Liquid
    For Use With (Application) Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
    Quantity 1000mL
    Storage Requirements Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture

    FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

    • Formulation without hypoxanthine and thymidine for use with or without dhfr systems
    • Ability to support high-density suspension growth and transient transfection of CHO-S cells
    • Animal origin-free, protein-free and chemically defined formulation
    • Easy use as part of the FreeStyle MAX CHO Expression System for protein production
    • Scalability in spinner flasks and bioreactors

    Formulation without hypoxanthine and thymidine for use with or without dhfr systems

    • Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
    • Non-dhfr systems require the addition of HT Supplement before use
    Ability to support high-density suspension growth and transient transfection of CHO-S cells
    • Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
    • Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
    • Cells usually require adaptation from serum-containing formulations
    • Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent
    Animal origin-free, protein-free and chemically defined formulation
    • Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
    • Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
    Scalability in spinner flasks and bioreactors
    • Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
    • The appropriate spinner or impeller speed and seeding density should be optimized for each system
    • Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture

    Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

  • Description & Specifications

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