Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Designed for serum-free single-cell plating of Chinese hamster ovary cells

Manufacturer: Gibco 31765092

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Catalog No. 31-765-092

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Required Accessories : Supplementation

    Specifications

    Format Liquid
    Formulation Biotin, Calcium Chloride (CaCl2-2H2O), Choline chloride, Cupric sulfate (CuSO4-5H2O), D-Calcium pantothenate, D-Glucose (Dextrose), Ferric sulfate (FeSO4-7H2O), Folic Acid, Glycine, Hypoxanthine, L-Alanine, L-Alanyl-L-Glutamine, L-Arginine hydrochloride, L-Asparagine (freebase), L-Aspartic acid, L-Cysteine hydrochloride, L-Glutamic Acid, L-Histidine hydrochloride-H2O, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Linoleic Acid, Lipoic Acid, Magnesium Chloride (MgCl2-6H2O), Niacinamide, Phenol Red, Potassium Chloride (KCl), Putrescine 2HCl, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4) anhydrous, Sodium Pyruvate, Thiamine hydrochloride, Thymidine, Vitamin B12, Zinc sulfate (ZnSO4-7H2O), i-Inositol
    Packaging Sustainable packaging
    Quantity 10 x 500mL
    Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
    With Additives Sodium Pyruvate; GlutaMAX™-I; Phenol Red

    Ham's F-12 Nutrient Mixture (F-12) has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells.

    • Contains zinc, putrescine, hypoxanthine, and thymidine
    • Serum-free growth of CHO cells in F-12 has led to variety of improved formulations
    • It is modified as follows: with GlutaMAX™, phenol red. Gibco F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in easy-to-use format
    • Contains no proteins or growth factors
    • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
    • Uses sodium bicarbonate buffer system (1.176g/L) and therefore requires 5-10% CO2 environment to maintain physiological pH
    • Sustainable packaging

    Cell Culture, Mammalian Cell Culture

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Description & Specifications

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