Mccoy's 5A (Modified) Medium Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Supports propagation of many types of primary cells, established cell lines, and explants from biopsy tissues

Manufacturer: Gibco 16600082

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Catalog No. 16-600-082

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Required Accessories : Supplementation


    Format Liquid
    Formulation Ascorbic Acid, Bacto-Peptone, Biotin, Calcium Chloride (CaCl2) (anhyd.), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Alanine, L-Arginine hydrochloride, L-Asparagine, L-Aspartic acid, L-Cysteine, L-Glutamic Acid, L-Glutamine, L-Histidine hydrochloride-H2O, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Nicotinic acid (Niacin), Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxal hydrochloride, Pyridoxine hydrochloride, Riboflavin, Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), Sodium Phosphate monobasic (NaH2PO4-H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
    Packaging Sustainable packaging
    Quantity 500mL
    Storage Requirements Storage: 2-8° C. Protect from light. Shelf life: 12 months from date of manufacture
    With Additives No Sodium Pyruvate; L-Glutamine; Phenol Red

    McCoy's 5A (modified) Medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues. This McCoy's 5A is modified as follows: with high glucose, L-glutamine, bacto-peptone, phenol red; without sodium pyruvate, HEPES.

    • Modification of Basal Medium 5A
    • Contains reducing agent glutathione, bacto-peptone, and a high level of glucose
    • Includes Dr. Hsu's addition of Hank's salts to enable use outside CO2 incubator
    • Requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS)
    • Uses sodium bicarbonate buffer system (2.2g/L), and therefore requires 5-10% CO2 environment to maintain physiological pH
    • Sustainable packaging

    Cell Culture, Mammalian Cell Culture

    This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

  • Description & Specifications

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