RPMI 1640 Medium, Powder Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

Originally developed to culture human leukemic cells in suspension and as a monolayer

Manufacturer: Gibco 31800089

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Catalog No. 31-800-089

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Required Accessories : Supplementation


    Format Powder
    Formulation Biotin, Calcium nitrate (Ca(NO3)2 4H2O), Choline chloride, D-Calcium pantothenate, D-Glucose (Dextrose), Folic Acid, Glutathione (reduced), Glycine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cystine 2HCl, L-Glutamic Acid, L-Glutamine, L-Histidine, L-Hydroxyproline, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Proline, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt dihydrate, L-Valine, Magnesium Sulfate (MgSO4-7H2O), Niacinamide, Para-Aminobenzoic Acid, Phenol Red, Potassium Chloride (KCl), Pyridoxine hydrochloride, Riboflavin, Sodium Chloride (NaCl), Sodium Phosphate dibasic (Na2HPO4-7H2O), Thiamine hydrochloride, Vitamin B12, i-Inositol
    Quantity 10L
    Storage Requirements Storage: 2-8° C. Shelf life: 36 months from date of manufacture
    With Additives No Sodium Pyruvate; L-Glutamine; Phenol Red

    Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

    • Modified as follows: with L-glutamine, phenol red; without HEPES, sodium bicarbonate
    • Contains reducing agent glutathione and high concentrations of vitamins
    • Contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium
    • Vitamins inositol and choline are present in high concentrations
    • Contains no proteins, lipids, or growth factors; therefore, requiring supplementation, commonly with 10% Fetal Bovine Serum (FBS)
    • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH
    • Powder forms of Gibco cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

    Cell Culture, Mammalian Cell Culture

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