Gibco Viral Production Cells are a clonal derivative of the HEK 293F cell line and are a core component of LV-MAX Lentiviral Production System. Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium. They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.
- Suspension culture cells facilitate high-throughput screening or scale-up
- Higher viral production levels than other suspension HEK 293 cells
- Rapid cell growth and high culture viabilities
Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.
The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.
|Viral Production Cells|
|Suspension Cell Culture|
|Store in liquid nitrogen until ready to use. Do not store the cells at −80°C.|
For Research Use Only. Not for use in diagnostic procedures.
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