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GL7 Monoclonal Antibody (GL-7 (GL7)), PE, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen 12590282

Encompass_Preferred

Catalog No. 50-112-9506

  • $269.65 / Each of 1

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    Reg : $280.00

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Description

Description

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis. Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analyis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

GL7
Monoclonal
0.2 mg/mL
PBS with 0.09% sodium azide; pH 7.2
Rat
100 μg
Primary
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
GL-7 (GL7)
PE
GL7 antigen; Ly77; Ly-77
Affinity chromatography
RUO
Human, Mouse
Antibody
IgM
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