DescriptionGLI-2 Polyclonal specifically detects GLI-2 in Human, Mouse, Rat samples. It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular).
|Western Blot 1:500-1:2000, Chromatin Immunoprecipitation 1:10-1:500, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular)|
|Cancer, Stem Cell Signaling Pathway|
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide|
|GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2|
|NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.|
|Western Blot, ChIP assay, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)|
|Human, Mouse, Rat|
|This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)|
|Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.|
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