GLI-2 Rabbit, Polyclonal, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 6 publications

Catalog Number	Mfr. No.	Quantity
NB600874SS	Novus Biologicals NB600874SS	0.025 mg
NB600874	Novus Biologicals NB600874	0.1 mg

Description

GLI-2 Polyclonal specifically detects GLI-2 in Human, Mouse, Rat samples. It is validated for Western Blot, Chromatin Immunoprecipitation, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Flow (Intracellular).

Specifications

• Antigen: GLI-2
• Dilution: Western Blot 1:500-1:2000, Chromatin Immunoprecipitation 1:10-1:500, ELISA 1:10000-1:30000, Immunohistochemistry 1:500-1:2000, Immunohistochemistry-Paraffin 1:10-1:500, Flow (Intracellular)
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Cancer, Stem Cell Signaling Pathway
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Alias: GLI family zinc finger 2, GLI-Kruppel family member GLI2, glioma-associated oncogene family zinc finger 2, HPE9tax helper protein 1, oncogene GLI2, Tax helper protein, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, THP, THP1, THP2, zinc finger protein GLI2
• Gene Symbols: GLI2
• Isotype: IgG
• Purification Method: Affinity Purified
• Test Specificity: NB600-874 is expected to cross-react with all 4 splice variants of Human Gli as the immunogen sequence is present in all 4. Tested by Western blotting on human lung, gives low background, but no band was detected.

• Concentration: 1 mg/ml
• Applications: Western Blot, ChIP assay, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Accession No.: P10070
• Gene ID (Entrez): 2736
• Immunogen: This affinity purified GLI-2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human GLI-2 (isoform a). (Uniprot: P10070)
• Primary or Secondary: Primary
• Content And Storage: Store at -20°C short term. Aliquot and store at -80°C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 167.78 kDa