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Invitrogen™ Glucagon Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHGCG

Encompass

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Catalog No. EHGCG


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Description

Description

The Human Glucagon (Hu GCG) ELISA quantitates Hu GCG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GCG. Principle of the method The Human GCG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.
Specifications

Specifications

Solid-phase sandwich ELISA
Colorimetric Microplate Reader
ELISA Kit
Biotin
P01275
2.5pg/mL to 130pg/mL
2641
RUO
2°C to 8°C
4 hr. 45 min.
Signal Transduction
<12%
96 Tests
Plasma, Serum, Supernatant
GLP1,GLP2,GRPP
96 assays
This ELISA pair antibody detects human Glucagon. Other species not determined.
GCPR
HRP
2.03-130 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 50 μL; Serum, 50 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
GLP1, GLP2, GRPP
<10%
Human
2.5 pg/mL
SDS
Documents
Product Certifications
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