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Goat anti-Human IgG, Fab2 Antibody, DyLight 649, Polyclonal, Novus Biologicals™

Goat Polyclonal Antibody

Manufacturer:  Novus Biologicals NBP151902

Catalog No. NBP151902


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Description

Description

IgG (H+L) Polyclonal antibody specifically detects IgG (H+L) in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
Specifications

Specifications

IgG
0.5 mg/ml
Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500
Affinity Purified
Goat
IgG
1 ml
Store at 4C. Do not freeze.
Polyclonal
Human
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin)
DyLight 649
PBS containing 0.2% BSA with 0.09% Sodium Azide
immunoglobulin heavy constant gamma 1 (G1m marker)
Human IgG-F(ab')2 Fragment
Immunogen affinity purified
RUO
Secondary
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.
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