Goat anti-Human IgG, Fab2 Antibody, DyLight 649, Polyclonal, Novus Biologicals
Goat Polyclonal Antibody
Manufacturer: Novus Biologicals NBP151902
DescriptionIgG (H+L) Polyclonal antibody specifically detects IgG (H+L) in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
|Flow Cytometry 1:50 - 1:200, Immunohistochemistry 1:50 - 1:500, Immunocytochemistry/Immunofluorescence 1:50 - 1:500, Immunohistochemistry-Paraffin 1:50 - 1:500|
|Store at 4C. Do not freeze.|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Paraffin)|
|PBS containing 0.2% BSA with 0.09% Sodium Azide|
|immunoglobulin heavy constant gamma 1 (G1m marker)|
|Human IgG-F(ab')2 Fragment|
|Immunogen affinity purified|
|Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. The antibody to human F(ab')2 was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with human F(ab')2. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with F(ab')2 from other species.|
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