Golgin-97 Mouse anti-Human, Clone: CDF4, eBioscience™ Invitrogen™

Mouse Monoclonal Antibody

Manufacturer:  LIFE TECHNOLOGIES 14976782

Catalog No. 14-976-782

Description

The monoclonal antibody CDF4 recognizes human Golgin-97. Golgin-97, a GRIP domain protein member that was first identified as a Golgi complex autoantigen associated with Sjogren's syndrome. Golgin-97 is a peripheral membrane protein found on the trans face of the Golgi apparatus. The protein G family member, Arl1, mediates the binding of Golgin-97 to the Golgi membrane. Like all GRIP proteins, Golgin-97 functions in maintaining the integrity of the trans Golgi network. Golgin-97 has also been shown to function as a tethering molecule in the retrograde transport of proteins from the endosomes to the trans Golgi network. During poxvirus infection, Golgin-97 incorporates into virions and plays a role in viral replication and morphogenesis. Applications Reported: This CDF4 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, immunohistochemical staining, and immunocytochemistry. Applications Tested: This CDF4 antibody has been tested by immunocytochemistry of fixed and permeabilized HeLa cells and can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Golgin-97 Monoclonal antibody specifically detects Golgin-97 in Human samples. It is validated for Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot

Specifications

• Antigen: Golgin-97
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q92805
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: CDF4
• Conjugate: Unconjugated
• Gene: GOLGA1
• Gene Alias: 0710001G09Rik; 2210418B03Rik; AW107649; gap junction protein, alpha 4, 37kD; Golga1; golgi autoantigen, golgin subfamily a, 1; Golgi97; golgin A1; Golgin subfamily A member 1; Golgin-97
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2800
• Product Type: Antibody
• Isotype: IgG1 κ