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Invitrogen™ gp130 (IL 6ST) Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMIL6ST

Encompass

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Catalog No. EMIL6ST


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Description

Description

The Mouse gp130 (IL-6ST) ELISA quantitates Ms gp130 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms gp130. Principle of the method The Mouse gp130 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Specifications

Specifications

This ELISA antibody pair detects human mouse gp-130. Other species not determined yet
ELISA Kit
Solid-phase sandwich ELISA
Cytokines & Receptors
Q00560
40pg/mL to 10000pg/mL
16195
RUO
2°C to 8°C
4 hr. 45 min.
Oncology
<12%
96 Tests
Plasma, Serum, Supernatant
5133400A03Rik,AA389424,BB405851,CD130,D13Ertd699e,gp130
96 assays
Colorimetric Microplate Reader
Biotin
HRP
40.96-10,000 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 3 μL; Serum, 3 μL; Supernatant, 100 μL
1 hr. 20 min.
Mouse
5133400A03Rik, AA389424, BB405851, CD130, D13Ertd699e, gp130
<10%
Mouse
40 pg/mL
SDS
Documents
Product Certifications
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