Granulocyte Marker Mouse anti-Rat, Biotin, Clone: HIS48, eBioscience™ Invitrogen™
Mouse Monoclonal Antibody
The HIS48 monoclonal antibody reacts with a molecule expressed on the surface of all rat granulocytes as well as monocytes. The antigen recognized by HIS48 is also expressed by erythroid lineage cells during their developmental stages in the bone marrow. HIS48 is commonly used as a phenotypic marker for rat granulocytes when used in combination with other markers (such as CD11b in which granulocytes are CD11b high/Rat Granulocyte (His48) medium expressers) Applications Reported: The HIS48 antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIS48 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.Granulocyte Marker Monoclonal antibody specifically detects Granulocyte Marker in Rat samples. It is validated for Flow Cytometry
|Granulocytes; PML; PMN|
|4° C, store in dark, DO NOT FREEZE!|
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
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