Grim-19 Monoclonal Antibody (1A8), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 14993782

Catalog No. 50-138-97

Description

Description: This 1A8 monoclonal antibody reacts with human, mouse, and rat Grim-19. This 16 kDa protein is expressed in many human tissues, especially in the heart and skeletal muscle. Lower expression levels of Grim-19 can be observed in the liver, kidney, and brain. Originally described as a nuclear protein, reports indicate that Grim-19 is primarily localized to mitochondria as an essential component of complex I of the electron transport chain. Grim-19 expression can be induced by interferon-beta and all-trans-retinoic acid in tumor cell lines, leading to cell death. Finally, Grim-19 has been shown to bind the transactivation domain of STAT3 and inhibit its transcriptional activity. The molecular mechanisms of how Grim-19 regulates STAT3 within the cell remains under active investigation. Applications Reported: This 1A8 antibody has been reported for use in immunoblotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry. Applications Tested: This 1A8 antibody has been tested by immunoblotting of HeLa cell lysate. This can be used at less than or equal to 5 μg/mL. It is recommended that the antibody be titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

A novel gene associated with Retinoid-IFN-induced Mortality (GRIM) GRIM-19 gene was identified. Antisense expression of GRIM-19 confers a strong resistance against IFN/RA-induced death by reducing the intracellular levels of GRIM-19 protein. Overexpression of GRIM-19 enhances cell death in response to IFN/RA. GRIM-19 is primarily a nuclear protein whose expression is induced by the IFN/RA combination. These data indicate that GRIM-19 is a novel cell death-regulatory molecule.

Specifications

• Antigen: Grim-19
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9ERS2, Q9P0J0
• Gene Symbols: Ndufa13, Ndufa13-ps1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100911483, 314759, 51079, 67184
• Content And Storage: 4° C
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Clone: 1A8
• Conjugate: Unconjugated
• Gene: Ndufa13
• Gene Alias: 2700054G14Rik; AU022060; B16.6; CDA016; Cell d; Cell death regulatory protein GRIM-19; cell death-regulatory protein GRIM19; CGI-39; CI-B16.6; complex I B16.6 subunit; complex I-B16.6; FLJ58045; FLJ59191; gene associated with retinoic and IFN-induced mortality 19 protein; gene associated with retinoic and interferon-induced mortality 19 protein; Gene associated with retinoic-interferon-induced mortality 19 protein; genes associated with retinoid-IFN-induced mortality 19; GRIM19; GRIM-19; LOC100911483; mitochondrial NADH:ubiquinone oxidoreductase B16.6 subunit; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-like; NADH:ubiquinone oxidoreductase subunit A13; NADH-ubiquinone oxidoreductase B16.6 subunit; Ndufa13; YjeF N-terminal domain-containing protein 3; YjeF_N3; Yjefn3
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2b

For Research Use Only.