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Invitrogen™ Growth Hormone Human ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EHGH1

Encompass

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Catalog No. EHGH1


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Description

Description

The Human Growth Hormone (Hu GH) ELISA quantitates Hu GH in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GH. Principle of the method The Human GH solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Human growth hormone (hGH) is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The GH1 gene, along with 4 additional related genes, is located at the growth hormone locus on chromosome 17. The five genes share a remarkably high degree of sequence identity. Alternative splicing results in 5 isoforms ranging from approximately 19 kDa to 24 kDa. HGH is produced by the somatotroph cells in the pituitary. It acts through binding to membrane receptors that belong to the cytokine receptor subfamily. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. Mutations or deletions in the gene lead to growth hormone deficiency and short stature.
Specifications

Specifications

Colorimetric Microplate Reader
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Growth Factors & Receptors
Solid-phase sandwich ELISA
P01241
4pg/mL to 600pg/mL
2688
RUO
2°C to 8°C
4 hr. 45 min.
Biomarker-Related Pathways
<12%
96 Tests
Plasma, Serum, Supernatant
GH,GH-N,GHB5,GHN,IGHD1B,hGH-N
ELISA Kit
96 assays
Biotin
HRP
2.5-600 pg/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Plasma, 25 μL; Serum, 25 μL; Supernatant, 100 μL
1 hr. 20 min.
Human
GH, GH-N, GHN, IGHD1B, hGH-N
<10%
Human
4 pg/mL
SDS
Documents
Product Certifications
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