H-2Dd Mouse anti-Mouse, Brilliant Violet 786, Clone: 34-2-12, BD Optibuild™
Mouse Monoclonal Antibody
Manufacturer: BD Biosciences 742465
The 34-2-12 antibody (also known as 34-2-12S) recognizes theα3 domain of the H-2D[d]. The binding of the antibody to its epitope is independent of theα1 andα2 domains andβ2 microglobulin. It cross-reacts with cells of the C3H.LG/Ckc strain. Reactivity with other haplotypes (eg, b, f, k, p, q, r, s /) has not been observed. Soluble mAb 34-2-12 blocks binding of the Ly-49A-expressing T lymphoma EL4 to immobilized H-2D[d]. However, further studies utilizing this mAb indicate that theα3 domain is not involved in the interaction between Ly-49A, or Ly-49G2, and H-2D[d].The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|(C57BL/6 x DBA/2)F1 mouse splenocytes|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV786 under optimal conditions that minimize unconjugated dye and antibody.|
|Brilliant Violet 786|
|Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
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