H3K4me1 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific 720072

Catalog No. PI720072

Description

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature. Since it is conserved across species, the antibody may react with many other species.

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post tranlationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specifications

• Antigen: Methyl-Histone H3 (Lys4)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HIST1H3A
• Gene Alias: H3K4me1, Histone H3, Histone 1, H3a, Histone H3.1, histone H3/a, HIST1H3A
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8350
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunofluorescence, Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.1% BSA, 30% glycerol and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P68431
• Gene Symbols: HIST1H3A
• Immunogen: Methylated peptide (Lys4) corresponding to Human H3 (aa 2-9)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG