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CD28 Hamster anti-Mouse, Brilliant Violet 711, Clone: 37.51, BD Optibuild™

Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 740670

Catalog No. BDB740670


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Description

Description

The 37.51 antibody reacts with CD28, which is expressed on most thymocytes, at low density on nearly all CD4+ and CD8+ peripheral T cells, and at even lower density on NK cells. The expression of CD28, in splenocytes and thymocytes, has been reported to increase after activation. CD28 transcripts are found in mast cells, and cell-surface expression of CD28 is induced upon maturation or activation of mast cells. It has been reported that CD28 is not expressed on some populations of intraepithelial T lymphocytes. CD28 is a costimulatory receptor; its ligands include CD80 (B7-1) and CD86 (B7-2). The 37.51 mAb augments proliferation and cytokine production by activated T and NK cells and can provide a costimulatory signal for CTL induction. There is considerable evidence that CD28 is a costimulatory receptor involved in many, but not all, T cell-dependent immune responses.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD28
37.51
Brilliant Violet 711
Aqueous buffered solution containing ≤0.09% sodium azide.
Hamster
IgG2, λ1
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
Cd28; CD28 antigen; T-cell-specific surface glycoprotein CD28
Affinity Purified
Mouse EL-4 (T-cell lymphoma) Cells
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
Documents
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