CD29 Hamster anti-Mouse, Brilliant Violet 650, Clone: HM β1-1, BD Optibuild™
Hamster Monoclonal Antibody
Manufacturer: BD Biosciences 740500
The HM β 1-1 monoclonal antibody specifically binds to the 130-kDa integrin β 1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin- α chains. With α 1 through α 6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with α v (CD51), it forms α α 8, and α 9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, epithelia, and oocytes. This hamster mAb to a mouse leukocyte antigen has been observed to crossreact with similar populations of rat leukocytes. Source of the immunogen was purified mouse VLA-4 ( α 4β 1, CD49d/CD29).
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.
|Brilliant Violet 650|
|Aqueous buffered solution containing ≤0.09% sodium azide.|
|Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.|
|Itgb1; Integrin beta-1; Fnrb; gpIIa; VLA-4 subunit beta|
|Purified mouse VLA-4|
|The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
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