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CD3e Hamster anti-Mouse, Brilliant Violet 650, Clone: 500A2, BD Optibuild™

Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 740461

Catalog No. BDB740461

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The 500A2 monoclonal antibody specifically binds to the 25-kDa ε chain of the T-cell receptor-associated CD3 complex expressed on mouse thymocytes, mature T lymphocytes, and NKT cells. Plate-bound and soluble forms of the 500A2 antibody can activate T cells in vitro . Activation of a mouse T-cell clone by the 500A2 antibody can be blocked by Fab fragments of the GK1.5 anti-CD4 antibody. This suggests that the 500A2 antibody may bind an epitope on CD3e close to a site at which CD4 associates with the T-cell receptor. The 500A2 antibody reportedly does not to crossreact with rat leukocytes.



Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
CD3; CD3 epsilon; Cd3e; CD3ε; T3e
Affinity Purified
Mouse T-cell receptor
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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