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CD69 Hamster anti-Mouse, Brilliant Violet 650, Clone: H1.2F3, BD Optibuild™

Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 740460

Catalog No. BDB740460


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Description

Description

The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits. CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages. CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear. H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN- γ-induced macrophage stimulation. IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.

Specifications

Specifications

CD69
H1.2F3
Brilliant Violet 650
Aqueous buffered solution containing ≤0.09% sodium azide.
Hamster
IgG1, λ3
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
Flow Cytometry
0.2mg/mL
VEA; Very Early Activation Antigen; AIM; Activation Induced Molecule
Affinity Purified
Mouse Dendritic Epidermal T Cell Line Y245
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
RUO
Primary
Murine
Documents
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