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CD80 Hamster anti-Mouse, Brilliant Violet 510, Clone: 16-10A1, BD Optibuild™

Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 740130

Catalog No. BDB740130

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The 16-10A1 antibody specifically recognizes CD80 (B7-1). This member of the Ig superfamily, along with CD86 (B7-2), participates in T-cell co-stimulation via interactions with CD28 and CD152 (CTLA-4). CD80 has been reported to be constitutively expressed on dendritic cells, monocytes, and peritoneal macrophages; and it is inducible on B cells by various means, including activation by LPS, IL-4, and the cross-linking of surface Ig. Expression of CD80 has been reported to be greatly enhanced on splenic B cells following activation by LPS, with peak expression occurring between 48 and 72 hours. It has been reported that the activation of purified B cells with LPS can induce CD80 expression in as few as 18 hours. The 16-10A1 antibody has been reported to block binding of CTLA-4 Ig to CD80 and to block T-cell activation by Con A-elicited peritoneal exudate cells and CD80-transfected cell lines. However, 16-10A1 antibody alone is not able to block T-cell activation by antigen-presenting cells. CD86 (B7-2) is an alternate ligand for CD28 and CD152 (CTLA-4). Preliminary reports indicate that the 16-10A1 mAb may block the binding of rat anti-CD80 mAb clone 1G10 (Cat. No. 553368). In addition, it has been reported that the 16-10A1 antibody may cross-react with an activation antigen expressed on IFN- γ-activated alveolar macrophages of the dog.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.



Brilliant Violet 510
Aqueous buffered solution containing ≤0.09% sodium azide.
IgG2, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Flow Cytometry
Cd80; B7; B7-1; Cd28l; Ly-53; MIC17; TSA1
Affinity Purified
Mouse CD80 (B7) Transfected Cell Line
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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