BioVision Inc HDAC Activity Fluorometric Assay Kit: Fluorometric HDAC Activity Assay Kit: Measure Histone Deacetylase (HDAC) Activity within an Hour. Convenient, High-Throughput Adaptable fluorescence-based Assay. 100 Assays.
Manufacturer: BioVision Inc K330100
HDAC Activity Fluorometric Assay Kit: Inhibition of histone deacetylase (HDAC) has been implicated to modulate transcription and induce apoptosis. However, screening compounds for HDAC inhibition has been difficult due to the lack of convenient tools for analyzing HDAC activity. The Fluorometric HDAC Activity Assay Kit provides a fast and fluorescence-based method that eliminates radioactivity, extractions, or chromatography, as used in traditional assays. The new procedure requires only two easy steps, both performed on the same microtiter plate. First, the HDAC substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g. nuclear extract) Deacetylation of the substrate sensitizes the substrate, so that further treatment with the Lysine Developer produces a fluorophore. The fluorophore can be easily analyzed using a fluorescence plate reader or a fluorometer. The assay is well suited for high throughput screening applications
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