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Thermo Scientific™ Lab Vision™ Heat Shock Factor 1 (HSF1) Ab-1, Rat Monoclonal Antibody

Ensure accurate, reproducible results in western blotting, immunofluorescence, immunoprecipitation and gel supershift experiments.

Manufacturer:  Thermo Scientific™ RT405P1

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Catalog No. RT405P1

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

Ensure accurate, reproducible results in western blotting, immunofluorescence, immunoprecipitation and gel supershift experiments with Thermo Scientific™ Heat Shock Factor 1 (HSF1) Ab-1, Rat Monoclonal Antibody.

HSF1 responds to cellular stress signals such as heat, heavy metals, and oxidative reagents; binds to heat shock response elements (HSEs) in the upstream region of heat shock gene and activates their transcription. Some laboratories have reported that the constitutive, non-DNA binding, monomeric form of inactive HSF1 is located in the cytoplasm. Upon activation, HSF1 is trimerized and is localized in nucleus where it binds DNA.

Host Species: Rat

Clone: 4B4

Isotype: IgG1

Species Reactivity: Human, Mouse and Rat. Others not known.

Epitope: aa 425-439 of mouse HSF1

Immunogen: Recombinant mouse HSF1 protein (aa 1-503)

Molecular Weight: 70-85 kDa (depending upon the source and state of cells)

Positive Control: LS174T or MAD109 cells

Cellular Localization: Nuclear

Recommended for:

  • Western Blotting
  • Immunofluorescence
  • Immunoprecipitation (Native only)
  • Gel Supershift

Note:

USA: RUO; Int'l: RUO

Specifications

Specifications

Heat Shock Factor 1 (HSF1) Ab-1
Monoclonal
200µg/mL
Concentrated
Recombinant mouse HSF1 protein (aa1-503)
500μL
Stress Response
Primary
Human, Murine
Immunoprecipitation, Western Blot
4B4
Unconjugated
Rat
IgG1
RUO
Primary
Monoclonal
SDS
Documents
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