HEF1 Monoclonal Antibody (2G9), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA15784

Catalog No. 01-674-895

Description

This antibody detects HEF1 in human, mouse, and rat samples. This antibody does not recognize p130cas. It has not been tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites.

HEF-1 is a multifunctional protein involved in integrin-based signaling that affects cell motility, growth, apoptosis and oncogenic transformation. The Cas family of docking proteins have been the subject of intense research because of their role in cell motility, growth, apoptosis and oncogenic transformation. These proteins are substrates of focal adhesion kinase (FAK) and the Src family of tyrosine kinases two active targets for drug development. HEF1 protein production increases levels of mRNA transcripts that encode proteins associated with motility, cell transformation and invasiveness, including several metalloproteinases, MLCK, p160ROCK and ErbBi. HEF1 overproduction also mediates apoptosis in epithelial-derived cell lines, including MCF7 and HeLa cells. Recent clinical studies at another institution have found that overexpression of BCAR1 (p130Cas), a related protein, is associated with tamoxifen resistance. This highlights the importance of studying the role of this family of proteins in cancer prognosis.

Specifications

• Antigen: HEF1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% sodium azide
• Gene Accession No.: O35177, Q14511
• Gene Symbols: NEDD9
• Immunogen: Fusion protein, corresponding to amino acids 82-398 of Human HEF1.
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot, Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot
• Clone: 2G9
• Conjugate: Unconjugated
• Gene: NEDD9
• Gene Alias: Cas scaffolding protein family member 2; CAS2; CASL; CAS-L; cas-like docking; CASS2; Crk-associated substrate lymphocyte-specific protein; Crk-associated substrate related protein Cas-L; CRK-associated substrate-related protein; dJ49G10.2; dJ761I2.1; Enhancer of filamentation 1; Enhancer of filamentation 1 p55; HEF1; mEF1; Nedd9; NEDD-9; Neural precursor cell expressed developmentally down-regulated protein 9; neural precursor cell expressed, developmentally down-regulated 9; neural precursor cell expressed, developmentally down-regulated gene 9; p105; p130Cas-related protein; renal carcinoma antigen NY-REN-12
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 18003, 291044, 4739
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov