HIF1A Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA585494

Catalog No. PIPA585494

Description

Keep as concentrated solution. Positive Control: MCF-7, HeLa(500uM CoCl for 24hr), NIH-3T3 (500 uM CoCl2 treatment for 24 hr), HepG2 whole cell lysate/extract (1% O2 treatment for 24hr), 293T transfected HIF1A, HepG2 ( 200 uM CoCl2 treatment for 24 hr), HepG2 (500 uM CoCl2 treatment for 24 hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

Specifications

• Antigen: HIF1A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: HIF1A
• Gene Alias: HIF-1alpha antibody/ HIF1 antibody/ HIF1-ALPHA antibody/ MOP1 antibody/ PASD8 antibody/ bHLHe78 antibody/ HIF1A antibody/ basic-helix-loop-helix-PAS protein MOP1 antibody/ class E basic helix-loop-helix protein 78 antibody/ ARNT-interacting protein antibody/ member of PAS superfamily 1 antibody/ hypoxia-inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor) antibody/ hypoxia-inducible factor 1-alpha antibody/ hypoxia-inducible factor 1 alpha isoform I.3 antibody/ PAS domain-containing protein 8 antibody/ member of PAS protein 1 antibody/ HIF-1-alpha antibody/ ARNT interacting protein antibody/ hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor) antibody
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 29560, 15251, 281814, 3091
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: ChIP assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
• Concentration: 0.26 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q61221, Q16665, O35800
• Gene Symbols: HIF1A
• Immunogen: Recombit protein encompassing a sequence within the C-terminus region of human HIF1 alpha.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG