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Cytiva His SpinTrap™ Spin Columns

Use for simple, small-scale purification of histidine-tagged proteins and rapid expression screening. Cytiva Life Sciences™ His SpinTrap™ Spin Columns include prepacked, single-use spin columns.

$669.00 - $682.00

Specifications

Description His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Type Spin Column Kit
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Products 2
Catalog Number Mfr. No. Type Price Quantity & Availability  
Catalog Number Mfr. No. Type Price Quantity & Availability  
45-001-427
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Cytiva
28401353
Spin Column Kit
Pack of 50 for $669.00
Add to Cart
 
45-002-017
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Cytiva
28932171
Spin Column Kit
Each for $682.00
Add to Cart
 
Description

Description

  • Single-use spin columns prepacked with Ni Sepharose High Performance Medium that is precharged with nickel ions; negligible ion leakage
  • One run in standard microcentrifuge takes approximatedly 10 min.
  • Yields up to 750μg purified protein per column
  • Allows direct purification of both clarified and unclarifed cell lysates
  • Also useful for screening large numbers of small-scale bacterial lysates
  • Kit option contains buffer, allowing fast and convenient purifications without time-consuming buffer preparation step

Medium Characteristics:

  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • Stable (long-term) in the pH range 3-12
  • Average particle size: 34μm

Order Info

Kit (28-9321-71) contains 50 columns and one pack of His Buffer Kit (2 x 100mL phosphate 8X stock solution, pH 7.4, and 100mL highly pure 2M imidazole, pH 7.4)

Specifications

Specifications

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)
6% Highly Cross-linked Agarose
4°C to 30°C
Spin Column Kit
SDS
Documents

Documents

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