MilliporeSigma™ His Tag Monoclonal Antibody

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Conjugate	Quantity
70-796-4MI	MilliporeSigma™ 707964	Unconjugated	3 μg
70-796-3MI	MilliporeSigma™ 707963	Unconjugated	100 μg

Description

The His Tag™ Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His Tag sequence encoded by many of the Novagen™ expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 x 10-8 to 1 x 10-9 M) enables sensitive, specific detection of His Tag fusion proteins at antibody concentrations of 0.1-0.2μg/ml. The His Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100μg package size provides enough purified antibody for 1000 ml of working solution.

Specifications

• Antigen: His Tag
• Classification: Monoclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Isotype: IgG1
• Content And Storage: -20°C

• Applications: Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Formulation: Lyophilized, BSA-free
• Primary or Secondary: Primary
• Test Specificity: HisHisHisHisHis, N-terminal, C-terminal or internal

For Research Use Only. Not for use in diagnostic procedures.