His tag Rabbit anti-Tag, liquid, TrueRAB™

Rabbit Recombinant Monoclonal Antibody

Supplier:  OriGene TA591008S

Catalog No. 50-167-7106

Description

Synthetic peptide sequence is HHHHHH

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombit proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombit protein using genetic engineering. By choosing a particular epitope and recombit protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombit proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.

Specifications

• Antigen: His tag
• Classification: Recombinant Monoclonal
• Conjugate: Unconjugated
• Gene Alias: ANTI HIS, ANTI HISTIDINE, ANTIHIS, anti-His, his tag antibody, Anti-His Antibody, his antibody, anti his tag, anti-his tag
• Immunogen: Synthetic peptide
• Regulatory Status: RUO
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid
• Target Molecule: Tag

• Applications: Western Blot
• Clone: OR-5B12
• Formulation: Tissue Culture Supernatant with 1% BSA and 0.02% sodium azide
• Host Species: Rabbit
• Quantity: 30 μL
• Primary or Secondary: Primary
• Product Type: Antibody
• Isotype: IgG