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Cytiva HiScreen™ Blue, Ni and DEAE Sepharose™ Prepacked Fast Flow Columns
Used for method optimization and parameter screening
Supplier: Cytiva 28978243
Description
- Based on 6% highly cross-linked agarose with an average bead size of 90μm
- Processed flow properties and high loading capacities
- Bed height of 10cm is designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
- Reproducible results, scalable to BioProcess™ columns packed with the same chromatography media using the same linear fluid velocity
- Bed volume: 4.7mL
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 90μm
- Flow velocity: <600cm/hr or <450cm/hr
- Material (Column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
Blue Sepharose
- Prepacked with BioProcess chromatography medium Blue Sepharose 6 Fast Flow
- Purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins
- Prepacked with Nickel Sepharose 6 Fast Flow
- Purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC)
- Prepacked with DEAE Sepharose Fast Flow
- Weak anion exchanger (diethylaminoethyl group) BioProcess™ medium
Specifications
70% Ethanol, 6M Guanidine Hydrochloric, 8M Urea | |
4.7mL | |
7.7mm | |
3.93 in. | |
<600cm/hr. | |
Cibacron Blue (7μM/mL medium) | |
4 to 12 | |
6% Cross-linked Agarose | |
Prepacked Fast Flow Column |
HiScreen Blue Sepharose FF column | |
0.3 in. | |
1 x 4.7mL | |
100mm | |
Polypropylene | |
1.5 bar | |
45 to 165μm | |
4° to 8°C | |
Purification of Albumin, Enzymes (including NAD+ and NADP+), coagulation facto rs, interferons and related Proteins |
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