Histone H3 (monomethyl K79) Rabbit, Clone: RM147, Abnova
Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human histone H3 (monomethyl K79).
Manufacturer: Abnova Corporation MAB12838
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
|Histone H3 (monomethyl K79)|
|Rabbit monoclonal antibody raised against synthetic monomethyl peptide of human histone H3 (monomethyl K79).|
|In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)|
|Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
|This antibody reacts to histone H3 monomethylated at Lysine 79. No cross reactivity with dimethylated Lysine 79 or trimethylated Lysine 79, or other methylations in histone H3.|
|ChIP assay, ELISA, Western Blot|
|ChIP (2-10 ug/mL) ELISA (0.2-1 ug/mL) Western Blot (0.2-1 ug/mL) The optimal working dilution should be determined by the end user.|
|A synthetic monomethyl peptide corresponding to residues surrounding K79 of human histone H3.|
|Protein A affinity purification|
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